In Osaka during the Edo period, ruler Sanada Yukimura was in losing position, fearing Toyotomi's blood descendents would end, he impregnates one of five Shinano female ninja with the seed of Toyotomi Hideyoshi in hopes of continuing the bloodline. Princess Sen and the five female ninja escape from Osaka castle by blending in with the handmaidens. Lord Tokugawa Ieyasu who was supposed to rule all under the heavens was informed of this secret. So Ieyasu orders five samurai of the Iga Ninja clan (brought by Hattori Hanzo) to stop the Toyotomi bloodline by killing the Kunoichi ninja. An attack of ninja magic arts unfolds in a battle of the sexes.
Yumiko Nogawa
Sanae Nakahara
Yuriko Mishima
Mari Yoshimura
Kyōsuke Machida
Shingo Yamashiro
Ryūji Shinagawa
Shoichi Ozawa
Shigeru Tsuyuguchi
Ryōsuke Kagawa
Yoshio Yoshida
Eizô Kitamura
Kokingo Ichikawa
Mitsuko Aoi
Katsumi Kaneko
Keiko Kojima
Miyuki Sasa
Ayako Satô
Minako Yamano
Akiko Matsushiro
Kinji Nakamura
Yoshihiro Igarashi
Kinnosuke Takamatsu
Hiroshi Hatano
Takashi Kaji
Ryōta Minowada
Meichō Soganoya
Michiyo Kogure
Minoru Ōki