She acted in more than 500 Tamil, Telugu, Malayalam, Kannada and Hindi movies. Jayamalini was born in Chennai on 22 December 1958 into a Tamil family. She was the youngest of 8 siblings. She was introduced to Tollywood in 1974 by Vittalacharya in the movie he produced, Aadadani Adrustam. She acted in several Telugu, Tamil, Kannada and Malayalam movies. She learned dancing from Heeralal master. She acted in Hindi movies too. She had a dance number in Shalimar (1978), starring Rex Harrison, Dharmendra and Zeenat Aman, which were in both English and Hindi versions. She also had a raunchy dance number, which was provocatively titled "Maamalu Maamalu Baavalu Baavulu", in the movie Maha Shaktimaan. Her sister Jyothi Lakshmi was also a cabaret dancer in films.
Annai Oru Alayam
Thai Meethu Sathiyam
Daana Veera Soora Karna
(Dancer)
Krodham
(Kamala)
Indradhanusu
Bahudoorapu Batasari
(Ananda Bhairavi)
Kudumbam
Idhu Enga Boomi
Veettukku Oru Kannagai
Valibamey Vaa Vaa
Vichitra Jeevitham
(Item number)
Superman
(Miss Lee)
Chambalkadu
Kudumbam Oru Koyil
Yamanukku Yaman
Sarithira Nayagan
Naam Iruvar
Driver Ramudu
(Kalavati)
Ramudu Kadu Rakshasudu
Iddaru Dongalu
Gopurangal Saivathillai
State Rowdy
Mahanagaramlo Mayagadu
Hero
Salem Vishnu
(Jaya)
Nakili Manishi
Annadammula Anubandham
Shalimar
Rani Kasula Rangamma
Guru
Gaja Donga
Chanakya Chandragupta
(Special Appearance)
Chandashasanudu
(Parijatham)
Tiger
(Special Appearance)
Aatagadu
(Special Appearance)
Magaadu
(Special Appearance)
Yugandhar
(Kamini)
Maha Shaktimaan
Gandharvya Kanya
Rahasya Rathri
Jaganmohini
(Jaganmohini)
24 Mani Neram
Andha Oru Nimidam
Maravil Thirivu Sookshikkuka
(Chinnamma)
Manavoori Pandavulu
Amara Deepam
Abkari
(Kanagam)
Kilikkonchel
Oka Radha Iddaru Krishnulu
Vetagadu
(Special Appearance)
Anbukku Naan Adimai
Malaiyoor Mambattiyan
(Sornam)
Yamagola
(Guest appearance)
Lok Parlok
Sainthadamma Sainthadu
Aattukara Alamelu
Maria, My Darling
Natchathiram
Dr. Siva
Sahodarara Savaal
(Parvathi)
Kudure Mukha
Muyyige Muyyi
(Dancer)
Vasantha Lakshmi
Kiladi Jodi
(Dancer)